ユーザーズガイド LEICA MM AF BROCHURE

[. . . ] ラ イ カ MM AF powered by MetaMorph ® Living up to Life 蛍光バイ オイ メ ージン グシステム MetaMorph MM AF MM AF MM AF / / MM AF 3D 3D MM AF 3D FRET FISH DMI6000 B 2 ─ラ イ カ MM AF MM AF MM AF CCD SuperZ Galvo I/O PIFOC MM AF DFC MM AF DFC 2 MM AF Offline MM AF SuperZ Galvo DFC360 FX 3 MM AF 2 XY パワフルな 多次元イ メ ージン グ ツール MM AF XYZ MM AF Z Z QuickTime® 3D Z AVI 2D 3D Continuous T cell receptor signaling required for synapse maintenance and full effector potential Johannes B. [. . . ] c 2003 National Academy of Sciences, U. S. A. syt A 5 m B syp syp A syt syp/ syp b 6 粒子 ト ラ ッキングと モーシ ョ ン解析 MM AF point-to-point X/Y Wendy C. Waterman-Storer, Department of Cell Biology and Institute for Childhood and Neglected Diseases, The Scripps Research Institute, La Jolla, CA 92037 MT F-actin (a) 3 http://www. jcb. org/cgi/ content/full/ jcb. 200203022/ DC1 Cy2 MT Xrhodamine F-actin Kymograph b-e lp lamellum la cz cb 3 MT Kymograph Actin (b-e) a Figure 3 leading edge 2 MT = 10 m Reproduced from The Journal of Cell Biology, 2002, 158(1), 31-37 by copyright permission of The Rockfeller University Press. 7 蛍光で要求される ス ピー ドと正確さ MM AF CCD MM AF MM AF FRET FRET Ran-GTP Petr Kalab, Karsten Weis, and Rebecca Heald, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200 Figure 4 Ran Ran-GTP 10 m (A) IYFP ICFP FRET MT-FRET YRC Ran-GTP FRET MT YIC IFRET/ICFP MT-FRET FRET FRET ICFP Reprinted with permission from Science (2002) 295(5564): 2452-2456. c 2003 American Association for the Advancement of Science. 8 カウン ト、 分類、 測定 多重細胞解析パラメ ーター MM AF / / MM AF 100 4 & Waltraud G. McNally, Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 Figure 5 a–f 3 GFP-GR a–f 113 a-f RNA FISH 1 m Reproduced from The Journal of Cell Biology, 2001, 154(1), 33-48 by copyright permission of The Rockfeller University Press. 1 RNA FISH RNA FISH 100 nM 9 技術情報 MM AF / DFC XY SuperZ Galvo EL6000 1 3rd Party Scan Slide Live Replay CCD &Z & EM CCD 3D 4D visualization & 3 Image stitching & MM AF / Mulitiwavelength Scoring 1 m U2OS Hoechst 33342 Anti-PhosphoTransfluor® Vesicles 7 2-RrGFP Histone H3 Ser28 mouse anti-beta tubulin primary antibody FITC FITC conjugated goat antimouse CHO-K1 secondary antibody Hoechst 33342 10 & & & MM Angiogenesis & QuickTime® & AVI MM Count Nuclei and Cell Scoring / MM Multi Wavelength Cell Scoring &Z Kymograph MM Cell Cycle IMA Integrated Morphometry Analysis Nearest neighbors & No neighbors 3D 2D FRET MM Neurite Outgrowth MM Cell Health MM Granularity MM Live Dead MM Mitotic Index MM Monopole Detection MM AF MM AF DM6000 / 11 Order nos. [. . . ] • LEICA and the Leica Logo are registered trademarks of Leica IR GmbH. [. . . ]